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NeuroScience Associates

Stroke Pathology: Ischemia Contrast

Applications:
  • This stain reveals the volume of affected brain tissue following ischemia.
Advantages:
  • Brains can be perfusion or immersion fixed.
  • Non-ischemic tissue remains intensely dark.
  • Affected tissue is pale, retaining less of the stain.
  • A density gradient is seen in marginally affected tissue, i.e. penumbra.
  • Other unstained, adjacent MultiBrain® sections are available for other stains.

Please note: This stain is performed exclusively by NSA

Specifications:

**NSA performs a digital analysis service on this stain as well.

Appearance of Stroke in Popular Animal Models With Decreasing Severity

Gerbil
Ligation of Carotid Arteries

4 N UMAX     PL-II            V1.5 [6]

4 N UMAX     PL-II            V1.5 [6]

4 N UMAX     PL-II            V1.5 [6]

Rat
Middle Cerebral Artery Occlusion

4 N UMAX     PL-II            V1.5 [6]

4 N UMAX     PL-II            V1.5 [6]

4 N UMAX     PL-II            V1.5 [6]

Abstract of Interest:

ISCHEMIA CONTRAST STAIN: A CLEAR WAY TO DELINEATE PENUMBRA FOLLOWING TRANSIENT FOCAL CEREBRAL ISCHEMIA

R.C. SWITZER III, PhD. NeuroScience Associates, Knoxville, TN 37922

H. KOENIG, MD. Dept. of Anesthesiology, Michael Reese Hospital,

Univ. of Illinois at Chicago, Chicago, IL 60610

Protective effects of interventions after stroke are ideally based on clinical neurologic outcomes and subsequent neurohistology. In small animal stroke models, difficulties with long survival times and neurologic evaluation prompt many investigators to perform acute studies and determine protection by histologic evaluations immediately after inducing an infarction. Many stains have been used to identify infarct areas but it is not clear if these areas include or could delineate the penumbra, i.e. the potentially salvageable tissue at the infarct’s border. The TTC method, for instance, is known to give variable infarct volumes depending on how long after the infarct the tissue is harvested. A fortuitous observation led to a staining method that delineates the penumbra and highlights the infarcted volume in sharp contrast. Formalin fixed brains from transient MCA-occluded rats were freeze sectioned (40µ), mounted and stained with a modified method for myelin. Normal tissue is dense black, infarcted areas fail to stain, and gradations of gray identify the penumbra zone. Section images are digitized and, based on optical density; the volumes of the infarct, penumbra and normal brain are calculated. Adjacent sections stained with other methods including H&E, thionine, and immuno-GFAP display less definition of affected areas.

The first published description of the results of this protocol was as an abstract for the 23rd Annual Meeting of the Society of Neurosurgical Anesthesia and Critical Care 1995.

Current contact information for Dr Heidi Koenig:

Dr Heidi Koenig
Anesthesiology-Conc. Care
530 S Jackson St
Louisville KY 40202-3617
hmkoen01@louisville.edu

For further reference, please see: Stains

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

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