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NeuroScience Associates

Immunohistochemistry (IHC) Staining

Click here to view our antibody gallery

Applications:

  • Antibody stains are used to detect the presence of specific biomolecules (antigens) in tissue sections by the application of an antibody to that antigen.

Advantages:

  • Provides unique staining to a given protein.

NSALabs® offers high quality immunohistochemistry staining services. Representative sample images of antibody stains performed by NSA are shown in our antibody gallery (click to view). NSA’s protocol for trials of new antibodies with our designated dilution series continually broadens the offerings we are able to make, including “in-house generated” antibodies.

New Antibody Trials/Titrations
NSA performs trials in small 12-well culture plates, using nine of the wells when performing our dilution series. The dilution series covers 8 dilutions plus one with no antibody. The dilution range is approximately in steps of one third from 1:150 to 500,000. That is: 1:150, 500, 1500, 5000, 15000, 50,000, 150,000, 500,000. NSA uses 2-4 ml per well of antibody solution. NSA’s vehicle solution is only TBS, with no BSA or other proteinaceous component. The lower viscosity of the solution is kinder to the sections. This follows the practice of Gloria Hoffman of University of Maryland, Baltimore. Once we establish the dilution, we process 10-20 large MultiBrain® sections per crystallizing dish (similar to a very deep petri dish), holding 60ml of solution. For previous and subsequent steps we use transfer baskets inside the crystallizing dish to minimize direct handling of the sections, thereby preventing tearing. Many of these implements can be found under NSA’s HistoTools®.

The antibody gallery is only a representative sample of antibodies we have performed. New antibody stains are performed weekly. If you do not see what you are looking for, please contact us or request a quote stating the antibody of interest.

Please note: Certain antibody stains require specialized protocols for perfusion and preparation of tissue. Consult with us prior to perfusion so that we may advise you on optimal handling. Contact us via our technical support form or call 865-675-2245.

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.