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NeuroScience Associates

Storing Free-Floating Sections

 

Antigen Preserve (AP) Solution

All MultiBrain sections are placed into AP storage solutions during sectioning. AP maximizes the antigenicity of proteins while eliminating concerns about formalin-sensitive staining which may be applied to the sections.

AP Solution Composition:
• PBS mixed as directed by manufacturer pH 7.0 – pH 7.4 with dH2O
• As a 10x concentrate, 1 part of liquid will be mixed with 9 parts of dH2O
• E.g. 100 ml of concentrate, 900 ml of dH2O
• Ethylene glycol
• Polyvinyl pyrrolidone (PVP)

Use these amounts or others in the same proportions, duly recorded, to make the required total volume:

Component2000 ml3000 ml4000 ml5000 ml6000 ml
10x PBS Concentrate100ml150 ml200 ml250 ml300 ml
dH2O900 ml1350 ml1800 ml2250 ml2700 ml
Ethylene glycol1000 ml1500 ml2000 ml2500 ml3000 ml
PVP20 g30 g40 g50 g60 g

The sections should be stored at -20° C for long term storage.

 

For more information:

Handling / Mounting Free-Floating Sections

Shipping Preparation for Cups of Free Floating Sections

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.