Storing Free-Floating Sections
Antigen Preserve Solution
For storage of cut sections in a manner that maximizes the antigenicity of proteins or for use with formalin-sensitive stains.
- PBS mixed as directed by manufacturer pH 7.0 –pH 7.4 with dH2O
- As a 10x concentrate, 1 part of liquid will be mixed with 9 parts of dH2O
- E.g. 100 ml of concentrate, 900 ml of dH2O
- Ethylene glycol
- Polyvinyl pyrrolidone (PVP)
Use these amounts or others in the same proportions, duly recorded, to make the required total volume:
|Component||2000 ml||3000 ml||4000 ml||5000 ml||6000 ml|
|10x PBS Concentrate||100ml||150 ml||200 ml||250 ml||300 ml|
|dH2O||900 ml||1350 ml||1800 ml||2250 ml||2700 ml|
|Ethylene glycol||1000 ml||1500 ml||2000 ml||2500 ml||3000 ml|
|PVP||20 g||30 g||40 g||50 g||60 g|
Prepare when depleted or expired.
Transferring Sections into Antigen Preserve Solution:
The sections will be cut and placed into the Antigen Preserve solution. The sections should be stored at <0° C.