Request a Quote          Contact Us          About NSA          Home       

NeuroScience Associates

Shipping Preparation for Cups of Free Floating Sections



From NSA to client:

Shipment of cups of free floating sections must be handled in a “very careful manner”, otherwise the sections will tear apart during transport. NSA is very experienced in this process, therefore our clients receive intact sections when we ship cups to them. The important step required for maintaining these intact sections during transport is TOPPING OFF (see video below).  That is, “overfilling” of the cups with the Antigen Preserve (AP) solution so that NO air remains within the cup when the lid is secured.  We check each cup after this filling by turning it upside down, observing for ANY air bubbles.  If one or more air bubbles are observed we remove the lid and “overfill” again, repeating this cycle until NO air bubbles are observed when turning over the cup.  We then tighten the lid more, parafilm the lid to the cup, and place in ziplock bags.

Topping Off Free-Floating Section Cups Video:


From Client to NSA or to collaborator:

Occasionally our clients return cups of unstained sections to NSA for additional staining, and/or they ship cups of sections to collaborators.  It is EXTREMELY important that the precautions above be performed before these shipments.  Because of the changes in temperatures from room temperature (when NSA sends the cups originally) and -20° temperatures which the sections have been stored, air bubbles are very likely to occur, even though the cups are intact since the original shipment.  It is important that the client follow the instructions above to assure safe shipment.  First, turn the cups upside down to observe for ANY air bubbles.  If none are observed the cup is ready for transport.  if, however there are ANY air bubbles, the lid must be removed, the cup must be TOPPED OFF with Phosphate-Buffered Saline (PBS) or dH2O (for the small volume that is required the AP solution isn’t required). See above for the complete description of this process.



For more information:

Storing Free Floating Sections

Handling / Mounting Free Floating Sections

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.


For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.