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NeuroScience Associates

Immunohistochemistry/Antibody Staining


Immunohistochemistry (IHC) is used to detect the presence of specific biomolecules (antigens) in tissue sections by applying an antibody to that antigen. IHC has become one of the most common tools in neurohistologic research due to the potential of high specificity when staining a target feature. NSA uses TritonX-100 to fenestrate membranes, allowing antibodies to stain the entire thickness of the section (30–80µ), removing thickness as a factor in NSA’s protocols.

We offer high-quality immunohistochemistry services utilizing commercially available antibodies and client-supplied proprietary antibodies and routinely perform titration series to expand our repertoire.

The following images are some of our most commonly requested immunohistochemistry.  Complete listing of commercial antibodies that NSA has successfully applied.

Examples of Successfully Performed Antibody Stains:



Fluorescent Immunohistochemistry/Antibody Stains


The gallery above represents the most common stains requested of NSA. If you don’t see a stain you desire, please contact us to determine if NSA can be of assistance.

For further reference, please see: Stains

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.


For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.