Immunohistochemistry/Antibody Staining

Immunohistochemistry/Antibody Stains

Immunohistochemistry (IHC) is used to detect the presence of specific biomolecules (antigens) in tissue sections by applying an antibody to that antigen. IHC has become one of the most common tools in neurohistologic research due to the potential of high specificity when staining a target feature. NSA uses TritonX-100 to fenestrate membranes, allowing antibodies to stain the entire thickness of the section (30–80µ), removing thickness as a factor in NSA’s protocols.

We offer high-quality immunohistochemistry services utilizing commercially available antibodies and client-supplied proprietary antibodies and routinely perform titration series to expand our repertoire.

The following images are some of our most commonly requested immunohistochemistry.  Complete listing of commercial antibodies that NSA has successfully applied.

Examples of Successfully Performed Antibody Stains:

4G8

6E10

Aβ1-40

Aβ1-42

Alpha Synuclein

Asyn-pSer129

AT8

Blood Brain Barrier

BrdU + hematoxylin

Calbindin

Caspase-3

Caspase-9

Cathepsin-D

CD68

c-fos

ChAT+Nissl

Doublecortin

Endoglin

Ferritin

GAD-67

GFAP

GFP

HuIgG

Iba1

Ki-67

LAMP1

Luciferase

MAP-2

MBP

MBP (degenerative)

mDectin

Nestin

NeuN

Olig2

Orexin A

Parvalbumin

p-c-jun

P.U.1

RGMa

S830

SMI-71

SMI-99

Somatostatin

STEM-101

TH

TDP-43

TMEM119

TPH