Immunohistochemistry/Antibody Staining
Immunohistochemistry/Antibody Stains
Immunohistochemistry (IHC) is used to detect the presence of specific biomolecules (antigens) in tissue sections by applying an antibody to that antigen. IHC has become one of the most common tools in neurohistologic research due to the potential of high specificity when staining a target feature. NSA uses TritonX-100 to fenestrate membranes, allowing antibodies to stain the entire thickness of the section (30–80µ), removing thickness as a factor in NSA’s protocols.
We offer high-quality immunohistochemistry services utilizing commercially available antibodies and client-supplied proprietary antibodies and routinely perform titration series to expand our repertoire.
The following images are some of our most commonly requested immunohistochemistry. Complete listing of commercial antibodies that NSA has successfully applied.
Examples of Successfully Performed Antibody Stains:
4G8
6E10
Aβ1-40
Aβ1-42
Alpha Synuclein
Asyn-pSer129
AT8
Blood Brain Barrier
BrdU + hematoxylin
Calbindin
Caspase-3
Caspase-9
Cathepsin-D
CD68
c-fos
ChAT+Nissl
Doublecortin
Endoglin
Ferritin
GAD-67
GFAP
GFP
HuIgG
Iba1
Ki-67
LAMP1
Luciferase
MAP-2
MBP
MBP (degenerative)
mDectin
Nestin
NeuN
Olig2
Orexin A
Parvalbumin
p-c-jun
P.U.1
RGMa
S830
SMI-71
SMI-99
Somatostatin
STEM-101
TH
TDP-43
TMEM119
TPH
