Antigen Preserve Solution
NSA places all sections directly into Antigen Preserve solution during the sectioning process. The storage of cut sections in this manner maximizes the antigenicity of proteins, as well as avoiding issues related to formalin-sensitive stains that may be performed on the sections.
Solution Composition:
- PBS mixed as directed by manufacturer pH 7.0 –pH 7.4 with dH2O
- As a 10x concentrate, 1 part of liquid will be mixed with 9 parts of dH2O
- E.g. 100 ml of concentrate, 900 ml of dH2O
- Ethylene glycol
- Polyvinyl pyrrolidone (PVP)
Use these amounts or others in the same proportions, duly recorded, to make the required total volume:
| Component | 2000 ml | 3000 ml | 4000 ml | 5000 ml | 6000 ml |
|---|---|---|---|---|---|
| 10x PBS Concentrate | 100ml | 150 ml | 200 ml | 250 ml | 300 ml |
| dH2O | 900 ml | 1350 ml | 1800 ml | 2250 ml | 2700 ml |
| Ethylene glycol | 1000 ml | 1500 ml | 2000 ml | 2500 ml | 3000 ml |
| PVP | 20 g | 30 g | 40 g | 50 g | 60 g |
Prepare when depleted or expired.
Transferring Sections into Antigen Preserve Solution:
The sections will be cut and placed into the Antigen Preserve solution. The sections should be stored at <0° C.
For more information:
