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NeuroScience Associates

Stroke

See Table of Stains appropriate for Stroke

 

 

Overview

Animal models for stroke attempt to induce a cerebral ischemia to mimic the effects of a stroke in humans which tends to be more difficult to replicate in smaller rodents than in larger animals. Neurohistology for stroke research primarily focuses on methods to quantify the ischemic region and characterize the margin of surviving tissue.


Animal Models
:

25 Mouse Brains Coronal

Up to 25 mouse brains are
co-embedded and appear on
each MultiBrain® slide section.

16 Rat Brains Coronal

Up to 16 rat brains are
co-embedded and appear on
each MultiBrain® slide section.

Dog Brain

Large brains such as a monkey,
sheep. and dog are processed
intact. If hemisected two
hemispheres are co-embedded
and appear on the same
MultiBrain® slide section.

Blood Brain Barrier (BBB) Breakdown

SMI-71 Endothelial Barrier Protein EBP
Rat-normal vessels

 

SMI-71 Endothelial Barrier Protein EBP Rat stroke
model reveals loss of EBP associated with
Blood Brain Barrier Compromise

Stroke Ischemia

Protective effects of interventions after stroke are ideally based on clinical neurologic outcomes and subsequent neurohistology. In small animal stroke models, difficulties with long survival times and neurologic evaluation prompt many investigators to perform acute studies and determine protection by histologic evaluations immediately after inducing an infarction. Many stains have been used to identify infarct areas, but it is not clear if these areas include or could delineate the penumbra, i.e., the potentially salvageable tissue at the margin of the infarct. The triphenyltetrazolium chloride (TTC) method, for instance, is known to give variable infarct volumes depending on how long after the infarct the tissue is harvested. A fortuitous observation led to a staining method that delineates the penumbra and highlights the infarcted volume in sharp contrast, hence the Ischemia Contrast Stain (ICS). Normal tissue is dense black, infarcted areas fail to stain and gradations of gray identify the penumbra zone. Section images are digitized and, based on optical density, the volumes of the infarct, penumbra and normal brain are calculated. Adjacent sections stained with other methods including H&E, thionine and GFAP IHC display less definition of affected areas.

Ischemia Contrast Stain (ICS)

Appearance of stroke in popular animal models with decreasing severity

Gerbil: Ligation of Carotid Arteries


Rat: Middle Cerebral Artery Occlusion






 

 

Image Analysis of Ischemic Area in Stroke Model

 

 

 

 

 

Gerbil brain section rendered ischemic from bilateral carotid ligation. The ischemia Contrast stain sharply delineates affected areas.

 

 

 

 

Once the image of the section is digitized, boundaries of the entire section and individual ischemic zones (1–3) are drawn, using density thresholding techniques with an NIH Image. Total volumes or an “index” of the volumes affected can be calculated from analysis on additional sections.
DATA:					  Area (sq. units)
 		Entire section			2.73
 		Area 1				0.02
 		Area 2				0.17
 		Area 3				0.11

                  % Ischemic area: 10.99%

If you would like further information about how NSA can assist you with your research, please contact us or refer to our catalog.

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.