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NeuroScience Associates

Stem Cell Detection

Applications:

  • Stem cells are introduced into a living host, often to achieve changes in the host’s composition. E.g. to replace missing/lost cells; replace cells with absent or faulty protein production.

Advantages:

  • The success of stem cell introduction is measured by a number of possible means but the initial goal is that the stem cells were placed in the correct location; that they survive and produce the changes intended. This assessment is accomplished by histologic examination of the hosts after different lapses in time after stem cell introduction.

Specifications:

  • Optimal fixation of the host brains by transcardial perfusion optimizes the conditions to ensure successful identification of the stem cells in the host animal.

 

Identification of Juvenile Neurons: Double Cortin IHC Reveals Neurons That Are <7 Days Old

 

Stem Cell Location

A fundamental measure of efficacy for stem cell treatments is to determine whether the stem cells reached the desired location in the central nervous system. Various immunohistochemistry probes can be used to detect signature markers of the stem cells (such as a unique human protein). Also stem cells can be deliberately made to produce markers such as β-Galactosidase, green fluorescent protein or others so that subsequent staining is able to highlight the location of introduced stem cells.

Cells were tagged with β-Galactosidase, revealed by
X-Gal histochemistry, demonstrates a successful
localization of the newly placed cells.

STEM 121 (human stem cells) in mouse spinal cord

 

Stem Cell Activity Example: Bromodeoxyuridine (BrdU) IHC

For some stem cell therapies, it is desirable for the cells to undergo a limited degree of division within the target organ. BrdU injection into the host animal (ip) before sacrifice (e.g. 6-12h) will allow the BrdU to be incorporated into newly formed DNA of dividing cells. BrdU masquerades for thmydine, which is only used in DNA synthesis. New cells created after the BrdU injection will contain BrdU which is detected and quantified with an antibody against BrdU.

The darkly stained cells (dots), mostly along the inner edge of
the “arrowhead” of the dentate formation indicate DNA
synthesis activity. In this manner, cell division can be witnessed
and quantified. The purple staining is normal cell body
background stained for anatomical reference.

 

 

 

For further reference, please see: Stains

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

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