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NeuroScience Associates

Parkinson Disease

See Table of Stains appropriate for PD

 

 

Overview

The primary pathological features of Parkinson Disease (PD) in humans are the loss of dopamine cells, the presence of Lewy bodies in existing cells and changes in the expression of alpha-synuclein. Disease models vary and NSA employs a wide range of tools suited to the traits of specific models.

Rodent TissueRodent brains are the primary tissue processed in PD Research by NSA. NSA can process up to 40 tissues in each MultiBrain® Block.

25 Mouse Brains Coronal

Up to 25 mouse brains are
co-embedded and appear on
each MultiBrain® slide section.

16 Rat Brains Coronal

Up to 16 rat brains are
co-embedded and appear on
each MultiBrain® slide section.

40 Mouse or 32 rat Brain
Hemispheres Coronal

Up to 40 mouse brains are
co-embedded and appear on
each MultiBrain® slide section.

Human Tissue:

NSA processes large format sections, as seen below, providing a unique opportunity to assess large contiguous cross-sections of tissue. NSA also processes multiple smaller samples from one or more brains using MultiBrain® Technology. The standard NSA practice of encasing the brain tissue in gelatin provides a significant aid in the handling of tissue sections resulting in an improved final product.

Human Brain Hemisphere

Multi-Embedded Human Brain Tissues

Tyrosine Hydroxylase (IHC)

PD research models typically use a method to induce the loss of dopamine cells or create lesions that interrupt the axons projecting to striatum. Tyrosine Hydroxylase is a robust marker of elements of the dopamine system.

Rat brain normal expressing TH equally
in both hemispheres.

Rat animal model treated to create the loss
of the dopamine cells that produce TH.

16 rat brains of a PD model on a MultiBrain® slide at the approximate
same levels. All brains have lost TH staining in the left striatum.

 

 

TH-Stained Substantia Nigra

AgNOR

Tyrosine Hydroxylase IHC

 

 

 

 

 

 

 

AgNOR-TH in Substantia Nigra

 

A silver nucleolar stain (AgNOR: Ag Nucleolar Organizing Region protocol used in oncology research), when paired with Tyrosine Hydroxylase IHC staining, is an excellent tool for use in stereology in Parkinson research.

 

 

Alpha-Synuclein

Alpha-Synuclein pSer129
Mouse Brain Cortex,10x

Alpha-Synuclein pSer129
Mouse Brain Cortex, 20x

Alpha-Synuclein pSer129
Synthetic A-Syn Fibril Injected
Mouse Brain Cortex, 20x

Alpha-Synuclein pSer129
A53T mouse cortex,
pyramidal cells

 

 

 

Alpha-Synuclein pSer129 Substantia nigra in a human PD brain, cytoplasmic and axonal aggregates, 60x

 

Placement of a vector containing the code for human alpha-synuclein in rat brains is taken up by cells and Alpha-Synuclein is expressed.

 

Transgenic mouse brain expressing human Alpha-Synuclein 211 (121-125), 20x (left) and 40x (right)

Disintegrative Degeneration

In PD research, models that employ an acute method of destroying the TH-producing cells, the degeneration of those cells can be witnessed using a Disintegrative Degeneration stain: Cupric Silver deOlmos method.

 

 

Degenerated neurons in substantia nigra in MPTP-treated BALB/C57 mouse

Inflammation

As with other disease models, inflammation markers such as GFAP and Iba1 can be useful indicators of perturbations in conjunction with other PD pathology.

 

Aberrant Iron in PD

Iron ‘decompartmentalized’ has been implicated in the pathologic process. Useful tools for probing this include Perls-DAB for ferric iron and Ferritin IHC for the iron storage molecule.

Perls-DAB reveals iron in the substantia nigra (left) and red nucleus (right) of this human tissue. This section was counterstained for nissl substance with Thionine (blue).

If you would like further information about how NSA can assist you with your research, please contact us or refer to our catalog.

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.