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Study Details

What is the time frame for this work?*

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1-6 months
1-3 years
Over 3 Years

Is this quote for a grant proposal?*

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Yes

Do you require GLP for this study?*

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No
Not sure/undetermined

Project Description*

Please describe your project in general (experiments, goals, anything that could impact the histology quality of service).

What species will you be using in your study?*

What type of tissue will you use for your study?*

Do you have a preferred staining frequency?

Standard staining frequencies for brains are every 6th for mice, every 8th for rats, every 24th for monkeys and every 33rd for humans.

How many animals are in this study?*

Would you like NSA to remove the brains/cords?*

Yes
No

Select the tissue orientation to section the tissue.*

What thickness would you like the tissue to be cut?*

NSA typically recommends 40µ for rat brains; 35µ for mice; 80µ for humans; no less than 25µ for any tissue; no less than 40µ for stereology.

Area of Interest (AOI):

Brain: Entire brain
Brain: Cerebrum (No Olfactory bulb; no cerebellum)
Brain: Hippocampus
Brain: Striatum
Brain: Striatum through substantia nigra
Cord: Entire cord
Cord: CTL (~10mm length each of cervical, thoracic, and lumbar)
Cord: Dot-Dash (Alternating coronal-horizontal segments for area specified below)

Additional Details for Area of Interest (AOI)?:

What animal model will you use? Please include genotype, vendor and stock number.

Please specify stains and/or antibodies:

Chromagen Preference:

DAB (brown color)
Nickel-DAB (blue-black color)
Immunofluorescence (please specify the fluorophores i.e. Alexa 488)

Cell population of interest:

Cell structure of interest (i.e. cytoplasm, nuclei, neuropil, synapsis, axons)

Would you like for NSA to perform any of the following additional services:

Digital Image Capture
Image Analysis
Pathology
Stereology

Injection sites: Are there any? If so, in which location? (If possible please include Bregma coordinates)

If necessary, can you provide NSA with positive control tissue? What type? Please provide as many details as you can (i.e. organ, animal, fixation)

Is there any literature related to your project that you are able to share with us or are trying to replicate? Please enter links below or email files to info@nsalabs.com.

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