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NeuroScience Associates

Acetylcholinesterase Enzyme Detection


  • Stain reveals acetylcholinesterase enzyme activity.


  • Allows different brain cell groups to be distinguished from others.


  • Tissue must be sent within 48 hours after perfusion.
  • After overnight in perfusion fix, then switch over to buffer and ship as soon as possible after that. This is an enzyme whose activity diminishes in time until the tissue is sectioned and stored in ‘antigen-enzyme’ preserve solution. Contact NSA to verify scheduling.
  • Follow perfusion instructions for immunohistochemistry stains on our website.

Acetylcholinesterase (AChE) Enzyme Staining

Macque monkey: AChE activity revealed with acetylthiocholine substrate and intensified with silver nitrate
(Hardy, H et al. Neuroscience Letters 3: 1/5, 1976)

Rat brain section stained for AChE
with the Hedreen method

Rat brain cortex stained for AChE
with the Hedreen method