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NeuroScience Associates

Acetylcholinesterase Enzyme Detection


  • Stain reveals acetylcholinesterase enzyme activity.


  • Allows different brain cell groups to be distinguished from others.


  • Tissue must be sent within 48 hours after perfusion.
  • After overnight in perfusion fix, then switch over to buffer and ship as soon as possible after that. This is an enzyme whose activity diminishes in time until the tissue is sectioned and stored in ‘antigen-enzyme’ preserve solution. Contact NSA to verify scheduling.
  • Follow perfusion instructions for immunohistochemistry stains on our website.

Acetylcholinesterase (AChE) Enzyme Staining

Macque monkey: AChE activity revealed with acetylthiocholine substrate and intensified with silver nitrate
(Hardy, H et al. Neuroscience Letters 3: 1/5, 1976)

Rat brain section stained for AChE
with the Hedreen method

Rat brain cortex stained for AChE
with the Hedreen method

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.


For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.