Request a Quote          Contact Us          About NSA          Home       

NeuroScience Associates

ß-GALACTOSIDASE (ß-GAL) Detection

Applications:

  • The X-Gal enzyme stain and the ß-Gal IHC method are tools to reveal ß-Gal presence.
  • These are used to identify the product of the lacZ gene, which is used as a marker for identifying cells that have been introduced into a host animal or transfected with a viral vector.

Advantages:

  • The signal observable from the enzyme method is usually considered superior to that achieved with the IHC method.
  • The enzyme method forms an intense blue precipitant in the presence of ß-Gal expression.
  • Tissue must be sent shortly after perfusion, as this is an enzyme whose activity diminishes with time.
  • Contact NSA to verify scheduling.

Specifications:

  • Tissue must be sent within 48 hours after the animal is perfused.
  • After perfusion and over night in fix, switch the brains to buffer (PBS) and then ship as soon as possible to NSA. This is an enzyme whose activity diminishes with time.
  • Contact NSA to verify scheduling.

X-Gal Enzyme Histochemistry

X-Gal Enzyme Histochemistry

 

 

β-Galactosidase IHC (β-Gal)

For further reference, please see: Stains

COVID-19 IMPACT: Our labs are currently OPEN for business. We are fully operational with uninterrupted delivery services ready to receive your tissue.

NSA IS OPEN

For clients with projects in process: NSA will communicate with you prior to shipping materials to ensure you are able to accept deliveries. If you are unable to receive deliveries due to the current global situation, NSA will hold shipments at our facility until your delivery system returns to normal. If you have not added digital imaging to your order but need it to expedite or continue your research, contact us right away so we can work together to meet your needs.

For clients with tissues, sections and/or slides located at our facility: Please allow us to help you continue your research, particularly if you are working remotely. We are able to perform additional stains, digitize slides for secure remote viewing, and perform additional analysis. Contact us today via email at info@nsalabs.com or using our contact form.

For new clients: We are ready to assist you with all of your neurohistology needs. We are open and fully operational with uninterrupted delivery services ready to receive your tissue.. This is a dynamic situation and we will update this message as things change.

NSA strives to minimize the impact of this global situation on your research and the neuroscience community as a whole. We wish you and yours the best as we maneuver through uncharted waters. Please contact us if we can assist you in any way.