High quality perfusion solutions yield high quality results. Commercially available formaldehyde solutions are NOT fresh and usually have added ingredients to ‘stabilize’ the solution for long shelf life. This should be considered a “technical grade” perfusion fix solution. Such solutions should only be used for standard, routine, classical stains. However, even these stains may be optimized by having a freshly prepared perfusion solution using reagent grade components.
For Disintegrative Degeneration Staining: Type 3 is required
FOR BEST RESULTS WITH MOST STAINS, USE PERFUSION SOLUTION TYPE 3. CONSULT WITH NSA PRIOR TO PERFUSION TO DETERMINE THE MOST APPROPRIATE SOLUTIONS TO USE.
Perfusion Solution Type #1: 'Technical Grade': Pre-made Phosphate buffered Formaldehyde
Perfusion Solution Type #2:‘Reagent Grade’: Phosphate Buffered Paraformaldehyde
Perfusion Solution Type #3:‘Superior Reagent Grade’: Na Cacodylate Based