PBS

Stock solutions:

  • Monobasic sodium phosphate: 0.2 M: 27.6 g / liter of dH20

  • Dibasic sodium phosphate (anhydrous): 0.2 M: 28.4 g / liter of dH20

Note: Be sure the dibasic is anhydrous and not hydrated; the formula weights will be different.

 

Phosphate-Buffered Saline Working Solution pH 7.2: 


  500 ml 1000 ml
Monobasic sodium phosphate 140 ml 280 ml
Dibasic sodium phosphate 360 ml 720 ml
Saline; NaCl 4.5 g 9 g

 

Sequence of Tissue Preparation Events with Links:

Fixation Method arrow Fix Solutions arrow Perfusion Instructions arrow Fix Timing & Storage Buffers arrow Shipping Tissue

 

Other Direct Links to Related Items:

Fixes:

Buffers:

Procedures:

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