When possible, the best neurohistologic results can be delivered when optimum procedures are followed for tissue preparation. For fixation, transcardial perfusion is the most effective fixation method and yields the best results. For some studies, perfusion is not possible, and an immersion fixation or "drop fix" can be acceptable for certain types of stains (such as basic stains and antibodies such as TH and GFAP).

Sequence of Tissue Preparation Events with Links:

Fixation Method Fix Solutions Perfusion Instructions Fix Timing & Storage Buffers Shipping Tissue

 

Other Direct Links to Related Items:

Fixes:

• Na Cacodylate Paraformaldehyde fix solution

• Paraformaldehyde fix solution

• Technical Grade, Phosphate buffered formalin fix solution

Buffers:

• Na Cacodylate buffer solution

• Phosphate Buffered Saline

Procedures:

• Post perfusion spinal cord preparation

 

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