For 1000 mL Total

1. Begin with 800 mL of dH2O on a magnetic stirrer.

2. Add 14.34 g Na Cacodylate and stir.

3. Bring the total volume to 1000 mL dH2O. Adjust the pH to 7.2–7.4 with concentrated HCl dropwise (ca. 15-20 drops).

Sequence of Tissue Preparation Events with Links:

Fixation Method Fix Solutions Perfusion Instructions Fix Timing & Storage Buffers Shipping Tissue

 

Other Direct Links to Related Items:

Fixes:

• Na Cacodylate Paraformaldehyde fix solution

• Paraformaldehyde fix solution

• Technical Grade, Phosphate buffered formalin fix solution

Buffers:

• Na Cacodylate buffer solution

• Phosphate Buffered Saline

Procedures:

• Post perfusion spinal cord preparation

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