Introduction | Scope of Stained Elements | Compare With H&E | Silver Degeneration Staining Evolution

Neonatal Cell Death (apoptosis) | Neurotoxicity Detection Paper | Planning a Neurotoxicity Experiment

Features Visible in the Disintegrative Degeneration Stain vs. the Traditional H&E Method:

The following images depict the same area of the brain on a control case and on a test article using MK-801 to create neurodegeneration. In the Disintegrative Degeneration (Amino Cupric Silver) stained section not only are the disintegrating cell bodies clearly visible, but the degenerating synaptic terminals, dendritic debris and axons are also conspicuous (see: Scope of stained elements). In the H&E stained section the damage is far less obvious and the characteristic eosinophilia of the cytoplasm and pyknotic nuclei of affected cells requires higher magnification to be distinct, as shown in the inset. In fact, if the observer did not know where to look, damage such as this might be overlooked in routine scanning of H&E slides.

Amino CuAg Control	Amino CuAg Affected	Amino CuAg Affected (16X)
H&E Control	H&E Affected	H&E (16X)

Introduction | Scope of Stained Elements | Compare With H&E | Silver Degeneration Staining Evolution

Neonatal Cell Death (apoptosis) | Neurotoxicity Detection Paper | Planning a Neurotoxicity Experiment

 

For futher reference, please see: Stains

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