Applications:

• Antibody stains are used to detect the presence of specific biomolecules (antigens) in tissue sections by the application of an antibody to that antigen.

Advantages:

• Provides unique staining to a given protein.

Specifications:

• Successful antibody staining may require different perfusion protocols and storage (of brains and sections) in different solutions. Consult with NSA prior to animal perfusion to determine the most appropriate protocol for a specific antibody.

NSALabs® offers high quality immunohistochemistry staining services. Below is a current listing of NSA's successful antibody staining (last updated September, 2004). As the list increases the website will reflect updates. Images of some of the representative antibody stains NSA has performed are shown below. NSA's protocol for trials of new antibodies with our designated dilution series continually broadens the offerings we are able to make, including “in-house generated” antibodies.

Please note: Certain antibody stains require special care and protocols during perfusion and preparation of specimens. If you plan to send us your animals, please consult with us prior to perfusion, if possible so that we may advise you on optimum handling. Email tech@nsalabs.com

Successfully Performed Antibody Stains: -3NT -4G8 -4HNE -Aß (1-42) -Aß (Pan; 1-40) -AT8 -anti-BACE-1 -ß-gal -BrdU -Caspase-3 -CD11b -CD-45 -c-fos -COX-2 -ED-1 -Gap-43 -GFAP -Glut 5 -nNOS -Orexin+ c-fos (double) -SMI 99 -SMI 32 -SMI 31 -Serotonin -Substance-P -Tyrosine Hydroxylase -VMAT2 -VAChT
	Tyrosine Hydroxylase-Rat
	COX-2 Rat Hippocampus	c-fos Rat Piriform Cortex
	GFAP- Mouse Amygdala Idiopathic Astrocyte Reactivity	GFAP- Rat Hippocampus Trimethyl Tin Intoxication
	Aß-1-40 (pan) Mouse Cortex PS-1/APP Transgenic	Aß-1-42 Mouse Cortex PS-1/APP Transgenic
	4G8 in Mouse PS-1/APP Transgenic	Orexin A antibody staining in rat hypothalamus
	Glut-5 for microglia; All microglia are stained. Changes in morphology distinguish resting microglia from reactive microglia (see upper left in left picture) Higher magnification on the right.

For futher reference, please see: Stains

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