Both the Campbell-Switzer method and the antibody stain for Aß amyloid are effective in revealing amyloid plaques as shown in the images below. The Campbell-Switzer method is capable of an additional dimension in that it stains a broad spectrum of plaque components including the mature/congophilic plaques (containing fibrillar amyloid) and the immature, so-called diffuse plaques containing pre- or beta-amyloid. Mature amyloid plaques stain amber while immature diffuse plaques stain black using the Campbell-Switzer Method. Supplementing normal plaque load analysis, the black and amber color differentiation allows the researcher an added dimension of analysis by being able to assess efficacy of a drug's ability to prevent diffuse plaques from attaining large sizes and/or reaching the amyloid state. The high contrast staining also makes the Campbell-Switzer stain highly amenable to imaging solutions when digital analysis is desired.

 

Campbell-Switzer Method	Antibody Stain for Aβ Amyloid
The Campbell-Switzer Method UNIQUELY allows researchers the ability to assess a drug's ability to prevent diffuse plaques from maturing.

 

Quantitative Analysis of Staining Results

NSA offers digital, systematic analysis of the stained sections from the Campbell-Switzer stain. For details, refer to Image Analysis.

 

For futher reference, please see: Stains

 

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